Research Groups

Nucleic Acids Structures and Repair

Secondary structures such as G-quadruplexes (G4s) can form within DNA or RNA. They pose a dramatic risk for genome stability, because due to their stability they can block DNA replication and this could lead to DNA breaks. In certain cancer cells mutations/deletions are observed at G4s, if a helicase that is important for G4 unwinding is mutated. Nevertheless, G4s are also discussed to be functional elements for cellular processes such as telomere protection, transcription, replication, and meiosis.
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  • Katrin Paeschke Visit
    Position

    Group Leader of the Laboratory of Nucleic Acids Structures and Repair

    Research fields

    Genome stability, genome regulation, DNA replication, DNA recombination, DNA damage and repair

    Postdoctoral Fellows
    Satya Pandey
    PhD Students
    Visit
    Enikő Fekete-Szücs
    Technicians
  • In order to better understand genome instability and the associated diseases and ageing in humans in greater details it essential to understand when, how, and why G4 structures form and what their impact is on genome stability. The aim of our research is to use various biochemical, genetical and computational tools to identify and characterize novel G4 binding protein and test their contribution on G4 formation and genome regulation. Our studies are performed using baker's yeast (Saccharomyces cerevisiae) and human cells.
    • Sauer M, Paeschke K.(2017) G-quadruplex unwinding helicases and their function in vivo. (Epub ahead of print)  
    • Wanzek K, Schwindt E, Capra JA, Paeschke K. (2017) Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability. Nucleic Acids Research.
    • Kazemier HG, Paeschke K, Lansdorp PM. (2017) Guanine quadruplex monoclonal antibody 1H6 cross-reacts with restrained thymidine-rich single stranded DNA. Nucleic Acids Research.
    • Benhalevy D, Sanjay K. Gupta, Danan CH, Ghosal S, Sun3 HW, Kazemier HG, Paeschke K, Hafner M, Juranek SA. (2017) The Human CCHC-type Zinc Finger Nucleic Acid-Binding Protein Binds G-Rich Elements in Target mRNA Coding Sequences and Promotes Translation. Cell Reports.  
    • Philips JA, Chan A, Paeschke K, Zakian VA. (2015) The Pif1 helicase, a negative regulator of telomerase, acts preferentially at long telomeres. PLoS Genetics.
    • Bochman ML, Paeschke K, Chan A, Zakian VA. (2014) Hrq1, a Homolog of Human RecQ4 Helicase, Acts Catalytically and Structurally to Promote Genome Integrity. Cell Reports.
    • Paeschke K, Bochman ML, Garcia PD, Cejka P, Friedman KL, Kowalczykowski SC, Zakian VA. (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature.
    • Bochman ML, Paeschke K, Zakian VA (2012) DNA secondary structures: stability and function of G-quadruplex structures. Nature Reviews Genetics.
    • Juranek S.A., Paeschke K. (2012) Cell Cycle Regulation of G-quadruplex DNA Structures at Telomeres. Current Pharmaceutical Design
    • Paeschke, K, Capra, J.A, Zakian V.A. (2011) DNA replication through G-quadruplex motifs is promoted by the S. cerevisiae Pif1 DNA helicase. Cell.
    • McGee, J.S., Phillips, J.A., Chan, A., Sabourin, M., Paeschke, K. and Zakian, V.A. (2010) Low Rif2 content marks short S. cerevisiae telomeres for elongation by telomerase while lack of Mec1 binding distinguishes them from double strand breaks. Nature Structural & Molecular Biology.
    • Capra, J.A., Paeschke, K., Singh, M. and Zakian, V.A. (2010) G-quadruplex DNA sequences are evolutionarily conserved and associated with distinct genomic features in Saccharomyces cerevisiae. PLoS Comput Biol.
    • Paeschke, K., McDonald, K.R. and Zakian, V.A. (2010) Telomeres: Structures in need of unwinding. FEBS Lett.
    • Schaffitzel, C., Postberg, J., Paeschke, K. and Lipps, H.J. (2010) Probing telomeric G-quadruplex DNA structures in cells with in vitro generated single-chain antibody fragments. Methods Mol Biol.
    • Paeschke, K., Juranek, S., Rhodes, D. and Lipps, H.J. (2008) Cell cycle-dependent regulation of telomere tethering in the nucleus. Chromosome Res.
    • Paeschke, K., Juranek, S., Simonsson, T., Hempel, A., Rhodes, D. and Lipps, H.J. (2008) Telomerase recruitment by the telomere end binding protein-beta facilitates G-quadruplex DNA unfolding in ciliates. Nat Struct Mol Biol.
    • Möllenbeck, M., Postberg, J., Paeschke, K., Rossbach, M., Jönsson, F. and Lipps, H.J. (2006) The end-replication machinery in spirotrichous ciliates. Endocytobiosis Cell Res.
    • Paeschke, K., Simonsson, T., Postberg, J., Rhodes, D. and Lipps, H.J. (2005) Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nat Struct Mol Biol.
    • Paschka, A.G., Jönsson, F., Maier, V., Möllenbeck, M., Paeschke, K., Postberg, J., Rupprecht, S. and Lipps, H.J. (2003) The use of RNAi to analyze gene function in spirotrichous ciliates. Europ J Protistol.
    • Mollenbeck, M., Postberg, J., Paeschke, K., Rossbach, M., Jonsson, F. and Lipps, H.J. (2003) The telomerase-associated protein p43 is involved in anchoring telomerase in the nucleus. J Cell Sci.
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