Research Sequencing Facility
Next generation sequencing (NGS) technology is revolutionizing medicine and life sciences and has become a routine tool to assess the genomes, epigenomes and transcriptomes of cultured cells, (liquid) biopsies, and primary tissue/disease samples. The Research Sequencing Facility, embedded in the ERIBA Technology Center, is a dedicated research infrastructure that provides support for NGS-based projects for UMCG research groups.
We will 1) facilitate the expeditious sequencing of NGS libraries either prepared by research groups themselves or by the facility on behalf of the researcher, 2) implement the latest NGS techniques used in medicine and life sciences research, and 3) (co)develop and implement new state-of-the-art NGS techniques to keep NGS-dependent research in the UMCG at the forefront.
We also advise the researchers on the set-up of their NGS experiments, e.g. which controls to include, which library prep kits or type of sequencing runs they can best choose for answering their specific research questions. We will also train researchers in the production of NGS libraries if they would prefer to do this themselves.
The facility aims to stimulate research and innovation by sharing their expertise, usage of available resources, increasing knowledge transfer and subsequently generating stronger interactions and collaborations between UMCG research groups.
CONTACT
If you are interested in making use of our services, please see the brochures below and use the Request Form.
D006 Sequencing at the Sequencing Facility v1-4
D007 NGS Library Quality and Quantity Control v1-4
F009 Checklist_for_sequencing_libraries_v2-1
For questions, please contact:
Email: research.sequencing.facility@umcg.nl
Post address: ERIBA, UMCG, Building 3226, PO Box 196, Internal Zip
Code FA50, 9700 AD Groningen
Telephone: +31 652724861
[[OUR SERVICES
The Research Sequencing Facility will accommodate single-cell DNA sequencing, single-cell and bulk RNA sequencing (small and large scale, including library preparation), small scale DNA sequencing projects and will implement new NGS protocols (e.g. ATAC-seq, END-seq, CROP-seq) by demand.
Generation and sequencing of a variety of NGS libraries
- Single-cell DNA template strand sequencing (Strand-seq)
- Single-cell whole genome sequencing (scWGS)
- Single-cell transcriptome sequencing (scRNA-seq)
- Mini-bulk (30 cells) whole genome sequencing (mini-bulk WGS)
- Mini-bulk (30-100 cells) transcriptome sequencing (mini-bulk RNA-seq)
- Transcriptome sequencing (RNA-seq)
- Whole genome sequencing (WGS)
- Whole exome sequencing (WES)
Single cell sorting
In our facility, we have a BD FACSJazzTM Cell Sorter available for plate-based single-cell sequencing projects for all researchers within the UMCG.
RNA/DNA quality and quantification
The Research Sequencing Facility has an Agilent 2100 Bioanalyzer available for quality control, sizing and quantification of RNA and DNA. Agilent offers a wide range of Labchip kits of which the facility has on stock the RNA 6000 Pico and DNA High Sensitivity kits (chips and all necessary reagents).
Large scale NGS projects
The Genome Analysis Facility (GAF) at the Department of Genetics will remain responsible for large scale (and outsourced) whole genome and exome sequencing projects, large scale DNA isolation and genotyping projects. Please contact Mathieu Platteel (m.platteel@umcg.nl) for more information or visit the GAF website (https://www.rug.nl/research/genetics/genomeanalysisfacility/).]]
[[OUR TEAM
d.c.j.spierings@umcg.nl
n.halsema@umcg.nl
j.beenen@umcg.nl
s.e.wobben@umcg.nl
r.arjaans@umcg.nl
l.kempe@umcg.nl
e.hiddingh@umcg.nl
]]
